Table 1.
Comparisons of RNA Expression Patterns of Tnnt 1 in Adult Brain Tissue of Mice that Received in vivo Exposures of Three CNS Stress Agents*
| Controls (Concurrent)§ | Ketamine | Radiation*** | Interferon-α | |
|---|---|---|---|---|
| Dose | n/a | 80 mg/Kg | 10 cGy, 200 cGy | 1x 105 IU/Kg |
| Sampling Time Post Treatment (Hour) | Concurrent | 0.5 | 4 | 4 |
| Exposure-induced Expression Patterns in: | ||||
| Choroid Plexus | Positive | Enhanced | Enhanced | Enhanced |
| Ependymal Lining of Ventricles | Positive | Enhanced | Enhanced | Enhanced |
| Hippocampus | ||||
| Ammon's Horn (CA1 - CA3 Regions) | Positive | Enhanced | Enhanced | Enhanced |
| Dentate Gyrus Region | Negative | Induced | Induced | Induced |
| Ammon’s Horn (A) vs Dentate Gyrus Region (DG) | A > DG | A > DG | A = DG | A > DG |
| Purkinje Cells of Cerebellum | Negative | Induced** | Induced | Not Induced |
| Pyramidal Neurons of Cereberal Cortex | Negative | Induced | Induced | Induced |
| Amygdala | Negative | Induced | Induced | Induced |
| Glial Cells | Negative | Not Induced | Not Induced | Not Induced |
Concurrent controls for ketamine and interferon received i.p. injection with distilled water; radiation controls were sham exposed.
Concurrent controls for all three stressors showed similar expression patterns. n/a: not applicable; positive: expression detectable in unexposed; negative: not detectable
Implication of RNA expression patterns of Tnnt 1 change: from weak to strong as "enhanced"; from negative to positive as "induced".
About 200 Purkinje cells per animal were scored, cells with Tnnt 1 stain scored as induced.
Comparison was made between the ketamine-exposed and control animals, p < 0.0001 [31]
Similar response in 10 cGy and 200 cGy animals, compared to control.
Modified from NeuroToxicology 30 (2009) 261–268, Lowe et al., Table 1, [32].