Abstract
The sequence of the 3'-terminal 21 nucleotides of 17S ribosomal RNA from the yeast Saccharomyces carlsbergensis has been determined to be (Y)G-m62A-m62A-C-U-C-G-C-G-G-A-A-G-G-A-U-C-A-U-U-AOH. This sequence shows extensive homology with the 3'-terminal sequence of 16S rRNA from Escherichia coli including the presence of the two adjacent N6-,N6-dimethyladenosines observed in the small subunit rRNA of eukaryotes as well as of many prokaryotes.
Full text
PDF








Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baan R. A., Hilbers C. W., Van Charldorp R., Van Leerdam E., Van Knippenberg P. H., Bosch L. High-resolution proton magnetic resonance study of the secondary structure of the 3'-terminal 49-nucleotide fragment of 16S rRNA from Escherichia coli. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1028–1031. doi: 10.1073/pnas.74.3.1028. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baralle F. E. Complete nucleotide sequence of the 5' noncoding region of rabbit beta-globin mRNA. Cell. 1977 Apr;10(4):549–558. doi: 10.1016/0092-8674(77)90088-5. [DOI] [PubMed] [Google Scholar]
- Brand R. C., Klootwijk J., Van Steenbergen T. J., De Kok A. J., Planta R. J. Secondary methylation of yeast ribosomal precursor RNA. Eur J Biochem. 1977 May 2;75(1):311–318. doi: 10.1111/j.1432-1033.1977.tb11531.x. [DOI] [PubMed] [Google Scholar]
- Brownlee G. G., Sanger F. Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose. Eur J Biochem. 1969 Dec;11(2):395–399. doi: 10.1111/j.1432-1033.1969.tb00786.x. [DOI] [PubMed] [Google Scholar]
- Dahlberg A. E., Dahlberg J. E. Binding of ribosomal protein S1 of Escherichia coli to the 3' end of 16S rRNA. Proc Natl Acad Sci U S A. 1975 Aug;72(8):2940–2944. doi: 10.1073/pnas.72.8.2940. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dasgupta R., Shih D. S., Saris C., Kaesberg P. Nucleotide sequence of a viral RNA fragment that binds to eukaryotic ribosomes. Nature. 1975 Aug 21;256(5519):624–628. doi: 10.1038/256624a0. [DOI] [PubMed] [Google Scholar]
- De Jonge P., Klootwijk J., Planta R. J. Terminal nucleotide sequences of 17-S ribosomal RNA and its immediate precursor 18-S RNA in yeast. Eur J Biochem. 1977 Jan;72(2):361–369. doi: 10.1111/j.1432-1033.1977.tb11260.x. [DOI] [PubMed] [Google Scholar]
- Ehresmann C., Stiegler P., Mackie G. A., Zimmermann R. A., Ebel J. P., Fellner P. Primary sequence of the 16S ribosomal RNA of Escherichia coli. Nucleic Acids Res. 1975 Feb;2(2):265–278. doi: 10.1093/nar/2.2.265. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eladari M. E., Galibert F. Sequence determination of 5'-terminal and 3'-terminal T1 oligonucleotides of 18-S ribosomal RNA of a mouse cell line (L 5178 Y). Eur J Biochem. 1975 Jun 16;55(1):247–255. doi: 10.1111/j.1432-1033.1975.tb02157.x. [DOI] [PubMed] [Google Scholar]
- Hunt J. A. Terminal-sequence studies of high-molecular-weight ribonucleic acid. The 3'-termini of rabbit reticulocyte ribosomal RNA. Biochem J. 1970 Nov;120(2):353–363. doi: 10.1042/bj1200353. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Khan M. S., Maden B. E. Nucleotide sequences within the ribosomal ribonucleic acids of HeLa cells, Xenopus laevis and chick embryo fibroblasts. J Mol Biol. 1976 Feb 25;101(2):235–254. doi: 10.1016/0022-2836(76)90375-2. [DOI] [PubMed] [Google Scholar]
- Klootwijk J., van den Bos R. C., Planta R. J. Secondary methylation of yeast ribosomal RNA. FEBS Lett. 1972 Oct 15;27(1):102–106. doi: 10.1016/0014-5793(72)80419-8. [DOI] [PubMed] [Google Scholar]
- Legon S. Characterization of the ribosome-protected regions of 125I-labelled rabbit globin messenger RNA. J Mol Biol. 1976 Sep 5;106(1):37–53. doi: 10.1016/0022-2836(76)90299-0. [DOI] [PubMed] [Google Scholar]
- McCutchan T. F., Gilham P. T., Söll D. An improved method for the purification of tRNA by chromatography on dihydroxyboryl substituted cellulose. Nucleic Acids Res. 1975 Jun;2(6):853–864. doi: 10.1093/nar/2.6.853. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Niles E. G. 5' -and 3'-terminal nucleotide sequences of Tetrahymena pyriformis 17S rRNA. Biochemistry. 1977 May 31;16(11):2380–2383. doi: 10.1021/bi00630a011. [DOI] [PubMed] [Google Scholar]
- Rosenberg M. Isolation and sequence determination of the 3'-terminal regions of isotopically labelled RNA molecules. Nucleic Acids Res. 1974 May;1(5):653–671. doi: 10.1093/nar/1.5.653. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shine J., Dalgarno L. Determinant of cistron specificity in bacterial ribosomes. Nature. 1975 Mar 6;254(5495):34–38. doi: 10.1038/254034a0. [DOI] [PubMed] [Google Scholar]
- Shine J., Dalgarno L. Identical 3'-terminal octanucleotide sequence in 18S ribosomal ribonucleic acid from different eukaryotes. A proposed role for this sequence in the recognition of terminator codons. Biochem J. 1974 Sep;141(3):609–615. doi: 10.1042/bj1410609a. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sprague K. U., Kramer R. A., Jackson M. B. The terminal sequences of Bombyx mori 18S ribosomal RNA. Nucleic Acids Res. 1975 Nov;2(11):2111–2118. doi: 10.1093/nar/2.11.2111. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Steitz J. A., Jakes K. How ribosomes select initiator regions in mRNA: base pair formation between the 3' terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli. Proc Natl Acad Sci U S A. 1975 Dec;72(12):4734–4738. doi: 10.1073/pnas.72.12.4734. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van de Voorde A., Contreras R., Rogiers R., Fiers W. The initiation region of the SV40 VP1 gene. Cell. 1976 Sep;9(1):117–120. doi: 10.1016/0092-8674(76)90057-x. [DOI] [PubMed] [Google Scholar]