Table 1.
AFLP primers used for bulked segregant analysis (BSA), genetic linkage mapping, and cluster analysis in N. langsdorffii.
| AFLP primer1 | DNA sequence (5′ → 3′) |
|---|---|
| E01 | AGACTGCGTACCAATTCA |
| M02 | GATGAGTCCTGAGTAAC |
| E32 | GACTGCGTACCAATTCAAC |
| E33 | GACTGCGTACCAATTCAAG |
| E36 | GACTGCGTACCAATTCACC |
| E39 | GACTGCGTACCAATTCAGA |
| E40 | GACTGCGTACCAATTCAGC |
| E41 | GACTGCGTACCAATTCAGG |
| M47 | GATGAGTCCTGAGTAACAA |
| M48 | GATGAGTCCTGAGTAACAC |
| M49 | GATGAGTCCTGAGTAACAG |
| M50 | GATGAGTCCTGAGTAACAT |
| M51 | GATGAGTCCTGAGTAACCA |
| M52 | GATGAGTCCTGAGTAACCC |
| M53 | GATGAGTCCTGAGTAACCG |
| M54 | GATGAGTCCTGAGTAACCT |
| M55 | GATGAGTCCTGAGTAACGA |
| M56 | GATGAGTCCTGAGTAACGC |
| M57 | GATGAGTCCTGAGTAACGG |
| M58 | GATGAGTCCTGAGTAACGT |
| M59 | GATGAGTCCTGAGTAACTA |
| M60 | GATGAGTCCTGAGTAACTC |
| M61 | GATGAGTCCTGAGTAAGTG |
| M62 | GATGAGTCCTGAGTAAGTT |
1All “E” primers were labeled at the 5′ end with WellRed D2 dye for fluorescent detection.