Table 3.
Primers for SSR marker amplification in Nicotiana spp., the NlRPT mapping population, and N. benthamiana*.
| Locus name | Tanneal (°C) | DNA sequence (5′ → 3′) | |
|---|---|---|---|
| GIS_A4* | F | 55 | TGCGCCAGTCAAATTCATACG |
| R | GGAGTCCGCAAGAGAGGAATA | ||
| GIS_A6 | F | 54 | TGGTGAAACAGTTGCCACAT |
| R | AGTCGCCTCAAGACTGAAACA | ||
| GIS_A9 | F | 53 | AACCCATGACAACGCATACA |
| R | TGGTCTATTTGCCACGTGAA | ||
| GIS_B3* | F | 55 | AGTCTATGCGAGGGGACCTT |
| R | TTGGTCCTGTGACTCCAACA | ||
| GIS_C12* | F | 53 | AAGAAATGGACAAACCAACTGC |
| R | CCTTGTTTGGGACTGAAACGTA | ||
| GIS_D1* | F | 54 | GATGCAGACCAGTTGCTGAA |
| R | TCAACCAACGCTGCAAGTAG | ||
| GIS_D3 | F | 52 | GCAGTTGTATATTTGAAAGCCAAA |
| R | TGCTGAAGGGTTAGATGTTCC | ||
| GIS_D7 | F | 52 | TCTTTTGGGCTTTCCACTTTTA |
| R | CTCGTGCCACAATATCATCAAT | ||
| KTRDC_B3 | F | 54 | GTGCAAACTGAACTGGCTGAC |
| R | TCAACATGGCTCGATAGATGG | ||
| KTRDC_B4 | F | 53 | TATGGCAATGTGCTTTGTATGC |
| R | TGCCATCATGAGATGTTTTTCA | ||
| KTRDC_B5* | F | 53 | CAAGCCATCCCATCTCCATA |
| R | GCCAGTGAGGACAAACGAGT | ||
| A104 | F | 54 | GGACAAAGTTAAGCCTACCCC |
| R | GGACTCCAATCGCTGTATCAA | ||
| A112* | F | 50 | CAATTACGATTTCTTTCCATCG |
| R | AAGTCACCTTGTTTGGTGTACG | ||
| A113* | F | 53 | GGACCATCTCAACAAAGATATGC |
| R | TGTTACAGTTTTGTGGTTTAGGG | ||
| A118* | F | 53 | GCATTTTCCCAAGAGACAGACTA |
| R | YTGGAAAGAAAAGAAGATGAGCA | ||
| A152 | F | 56 | TCCACAGCACAGACCCTTTACT |
| R | CTCACACACAAACACACACACG | ||
| A154 | F | 54 | TATCACCAACGCACTCATCTC |
| R | AGTCAGGCTTGTCAGCTCATT | ||
| A159* | F | 52 | CCCATAAAATTGGGGCCTTA |
| R | TGGAGTTGATGGTTGTTCCTG | ||
| A160 | F | 55 | ATGACGAGTGCCTGAGTCCTA |
| R | GCGTGTGACCTTATCAGCTTC | ||
| A161* | F | 53 | CCGCTAGTTGAATCCTCAATTCT |
| R | TGATATGATTACCCCTGGTGTTG | ||
| A172 | F | 53 | CACGTATTTGCCTGCCTAACA |
| R | CAAGGTCCCATATTGGGCTAA | ||
| A204* | F | 54 | CTTGGTCAACGGGTTCATCT |
| R | ATGTGGAACTGCGGTTATGC | ||
| A207 | F | 53 | TATTTCACCCACAGCCTTCTT |
| R | GTGTTCACCTGTGCCAATTAAA | ||
| A218* | F | 54 | TCGGCCTCACAGCAATAATC |
| R | CTCATCTCCCAGGCCCTAAA | ||
| A224* | F | 55 | CAGAGGATCATGCGACCACT |
| R | CCGCCTCTTGCACAAAGTAG | ||
| B151 | F | 54 | CCTTCCCTACTCAAGTTTCAATGT |
| R | AAACAGAGAACGCCAAATGGA | ||
| B155* | F | 55 | GTCGAGAGGGCTAAGTGAACAA |
| R | ACAAGTCATCGACACGCGACAA | ||
| B158 | F | 54 | AAGCCTTTTCTTTTTGAGGGACT |
| R | CTTCAATCTTCAAACCACCACTG | ||
| B162* | F | 55 | TGAGAAGGGGAAAAGGAGGTT |
| R | TACTCTCAGCGAGGTGACGAA | ||
| B202* | F | 52 | CAAGGTCTGCTATTGTGAGTG |
| R | CACCAGTGTTCCACGTAAAGAA | ||
| B211* | F | 54 | TGCTGTTGCAAGGTCTGCTAT |
| R | AAATCTGGAGTGCCCAATGTC | ||
| B215* | F | 55 | CCCAGTCTCCTTAGCCATCTG |
| R | AGGGCAGCATATCCGCTACT | ||
| C156* | F | 53 | CACAATATCCTGCCCATACCC |
| R | GAAGAAATTCCCGGCTTCAC | ||
| C157 | F | 53 | TCACTATTCTTGAGCCGAGGA |
| R | GTCATTCCTGTCGATTGATGC | ||
| C161 | F | 53 | TCACAAGATGGAACAGTACATGC |
| R | TCCAAAGACTTTTGTAGTGGTGA | ||
| C167 | F | 54 | CCTTGAGCCGAGGGTCTATT |
| R | GGGCAGGCAATTGAGATACA | ||
| C202* | F | 54 | TGGGATCACACTGGGTATGT |
| R | TCCATTCGGGTTACCTTTTCC | ||
| D152 | F | 53 | GCGAACGACATTTCGCTAAG |
| R | ATAGGGAACACGCGCAACAT | ||
| D159 | F | 54 | AATACCGAACCGAAATACCGAAT |
| R | AAGCGAACGACATTTGACTAAGG | ||
| D161 | F | 54 | GATGCAGACCAGTTGCTGAA |
| R | TCAACCAACGCTGCAAGTAG | ||
| D171 | F | 54 | TCGCATGAAAGAGACCTCGTA |
| R | CTCCGAAGGAACACATCCATT |