Table 4.
Primers used for PCR amplification of COSII, SCAR, ISSR, and TRAP markers in N. langsdorffii.
| Marker locus | Type | Tanneal (°C) | Restriction enzyme | DNA sequence (5′ → 3′) | |
|---|---|---|---|---|---|
| At1g02140 | COSII | F | 55 | HinFI | TCCGTTATGCTAACAATTCCAAC |
| R | TGTGTTCATTTCCCATCACAATCTC | ||||
| At1g30580 | COSII | F | 54 | ScrFI | TTCTGCCGAAGATTCATGCATGG |
| R | TCTCTCCACAGCAGCACTGAAAGG | ||||
| At1g60640 | COSII | F | 53 | DpnII | TCTGACAGGGGGAAATGAATCTTC |
| R | ACTTCATTGAAAGAGCTATCTTCACTCTC | ||||
| At2g16920 | COSII | F | 55 | TaqI | TGCAAGACAATGATGTATCTGATGAG |
| R | TCCTAAAGTGCTCTCTGATAAGCTCT | ||||
| At3g10220 | COSII | F | 54 | None | TGGCTTCTCAGTTACAGATTCAAGG |
| R | AACCTCCGGAGGTCACTGACG | ||||
| At4g21520 | COSII | F | 52 | None | TGACGGGGAGTCTGTATATGACTATTG |
| R | TGATTGCCCTTCTTTATTTCCTTG | ||||
| At5g37360 | COSII | F | 52 | DpnII | TGCAGCATTTGATCTTTCAAATGG |
| R | TGATTTTTGAGAGCCTGTCAATGAG | ||||
| At5g62390 | COSII | F | 54 | RsaI | TGCTACTAACTGTTGATGCCATTGAG |
| R | TTGGGGGTCGATAACATCAAGC | ||||
| At5g64350 | COSII | F | 53 | AvaI | AGATCGGCCAAGGCAAAGTTATC |
| R | TGCATGCCCAGTACTCCTTCATCC | ||||
| UBC825 | ISSR | 49 | None | ACACACACACACACACT | |
| UBC826 | ISSR | 51 | None | ACACACACACACACACC | |
| U826_900 | SCAR | F | 53 | None | ACACACACACACACACCAAAAGG |
| R | ACACACACACACACACCACTCTC | ||||
| U826_1400 | SCAR | F | 54 | DpnII | ACACACACACACACCCAGATTG |
| R | ACACACACACACACACCAGAAC | ||||
| U826_1900 | SCAR | F | 55 | None | ACACACACACACACACCGTGT |
| R | ACACACACACACACACCAAAAAA | ||||
| NBS91 | TRAP | 50 | TGTGGAGGRTTACCTCTAGC | ||
| Em3 | GACTGCGTACGAATTGAC | ||||
| ME2 | TGAGTCCAAACCGGAGC |
1Primer labeled with WellRed dye D3 at the 5′ end to enable fluorescent detection.