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. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: J Immunol. 2012 Jul 6;189(4):2017–2022. doi: 10.4049/jimmunol.1103623

Figure 1. Hemoglobin synergizes with HMGB1.

Figure 1

Bone marrow-derived macrophages (BMDMs) from C57BL/6 mice were washed 3 times with serum-free medium and then cultured overnight with HMGB1 at different concentrations alone or with mouse hemoglobin (30 μg/ml) (A, B), or HMGB1 at 4 μg/ml with mouse hemoglobin at different concentrations (C,D). Concentrations of TNF (A, C) or IL-6 (B, D) in the supernatants were determined by ELISA. The results represent mean ± s.e. and are representative of more than three independent experiments. * P < .05, ** P < .01, compared between cells treated with and without hemoglobin.