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. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: J Immunol. 2012 Jul 6;189(4):2017–2022. doi: 10.4049/jimmunol.1103623

Figure 6. Hemopexin suppresses the synergy induced by hemoglobin, HMGB1 and TLR agonist LPS or P3C.

Figure 6

BMDMs from C57BL/6 mice were washed 3 times with serum free medium and then cultured overnight with hemoglobin (Hb) (10 μg/ml), HMGB1 (2 μg/ml), LPS (250 pg/ml) (A) or Pam3Cys (P3C) (1ng/ml) (B) in the absence or presence of hemopexin (Hx) at indicated concentrations. Concentrations of TNF in the supernatants were determined by ELISA. The results denote the mean ± s.e and are representative of three independent experiments. ** P < .01, compared between cells treated with and without hemopexin.