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. Author manuscript; available in PMC: 2012 Aug 24.
Published in final edited form as: Cancer Res. 2010 Apr 20;70(9):3526–3536. doi: 10.1158/0008-5472.CAN-09-3278

Figure 2. Anti-proliferative and pro-apoptotic effects of sunitinib on MDSC subsets in the spleen.

Figure 2

(A,B) After 3, 6, and 9 days +/− sunitinib initiation to 4T1+ mice, mice were pulsed with BrdU (1 hour following sunitinib dosing) for 4 hours. Splenic and BM CD11b+Gr1+lo and CD11b+Gr1+hi cells were gated on and BrdU positivity determined by FACS. Cells from mice not given BrdU were used as staining controls. (C) 4T1+ splenocytes were stained for CD11b, Gr1, Ly6G, and F4/80 before and after MDSC subset isolation with magnetic beads. Representative FACS data and cytospins shown. n=2. (D) Mice were treated as above for (A) only splenocytes were stained for CD11b, Gr1, and annexinV and immediately ran for FACS.