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. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: J Membr Biol. 2012 Jul 15;245(7):401–409. doi: 10.1007/s00232-012-9462-2

Figure 4.

Figure 4

Effects of carbenoxolone treatment on YoPro-1 uptake induced either by mechanical stimulation caused by liquid displacement or by ATP application in (a) MC3T3-E1, (b) MOB-C, and (c) 43KO-MOB-C osteoblasts. For these studies, cells were bathed in 5μM YoPro-1 and effects of 20μM carbenoxolone (CBX) were evaluated 20 min after treatment. For the ATP stimulated group, 1mM ATP was added 10 min after CBX. All experiments were performed in low divalent cation PBS (LDPBS). Percent of YoPro-1 positive cells was calculated by normalizing dye positive cells with total number of cells in each image field x100. All data are presented as mean ± SEM, n = 3. P values were obtained using one way ANOVA analysis followed by Tukey’s multiple comparison test. (***P < 0.0005, ATP treated cells vs. all other treatments)