Fig. 6.

Alu antisense inhibits ERK1/2 activation induced by DICER1 knockdown and rescue cell viability in human RPE cells. Alu antisense (Alu-AS) transfection inhibits ERK1/2 phosphorylation induced by DICER1 knockdown assessed by Western blotting (A) and immunofluorescence (B). Alu-AS protects human RPE cells from cytotoxicity induced by DICER1 knockdown (C). Values are mean ± SEM, n = 3, *P < 0.05 by ANOVA and post hoc Newman–Keuls test. Images are representative of three independent experiments (A–C).