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. 2012 Jul 30;109(34):13704–13709. doi: 10.1073/pnas.1203126109

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Fig. 3. — Identification of centromeric epialleles on HSA17. (A) FISH for D17Z1 (red) and D17Z1-B (green) was combined with CENP-A immunostaining to determine the CENP-A location in the diploid line GM08828. CENP-A was located at D17Z1 on both homologs (H1 and H2). (B and C) CENP-A immunostaining and D17Z1/D17Z1-B FISH were performed on line FIBL1 (B) and line FIBL3 (C). In both lines, CENP-A was located at D17Z1 (red) on H1 but at D17Z1-B (green) on H2. (Scale bars: 5 microns in A–C.) (D) Extended chromatin fibers from line FIBL1 confirmed the CENP-A location (blue) at D17Z1 (red) on H1 and at D17Z1-B (green) on H2. (Scale bars: 15 microns for H1; 5 microns for H2).