Fig. 3.
Distance measurements for CaM residues: T35, T45, and T111. Superimposed currents recorded from oocytes injected with 3 μM T35C–Glyn–QA, T111C–Glyn–QA, or T45C–Glyn–QA protein are shown for each residue (Left). Currents were elicited with 4-s test potentials to +40 mV from a holding potential of -80 mV followed by a tail pulse to -30 mV. Dashed line indicates zero current. (Right) Normalized inhibition values plotted as a function of end-to-end linker length. Data are presented as the mean ± SEM from two to four batches of oocytes. The data were fitted to a Boltzmann equation to generate the midpoint of inhibition (d1/2): T35C, 40 ± 1 Å; T111C, 50 ± 1 Å; T45C, 49 ± 1 Å. Values are reported as mean ± the error of the fit.