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. 2012 Aug 7;109(34):13567–13572. doi: 10.1073/pnas.1207903109

Fig. 2.

Fig. 2.

SidF is required for anchoring SidC to the bacterial phagosomes. (A) Representative images of SidC immuno-staining of mouse bone marrow-derived macrophages infected with indicated L. pneumophila strains at an MOI of 1 for 1 h. (B) Quantitation of SidC positive bacterial phagosomes. Phagosomes positive for SidC staining are normalized against total phagosomes. Data shown are from two independent experiments performed in triplicate in which at least 100 phagosomes were scored per coverslip. **P < 0.01, paired Student t test. WT: L. pneumophila Philadelphia-1 wild type strain Lp02; dotA-: the type IV secretion system defective strain Lp03(dotA-); ΔSidF: the sidF deletion mutant Lp02 strain; ΔSidF(pSidF): the sidF deletion Lp02 strain complemented with a plasmid expressing SidF and ΔSidF(pSidF C/S): the sidF deletion Lp02 strain complemented with a plasmid expressing SidF C/S catalytically dead mutant.