Table 2.
BM |
Spleen |
|||
Colony type | KIT+/+ | KitV558∆;T669I/+ | KIT+/+ | KitV558Δ;T669I/+ |
BFU-E | 3,938 ± 1,042 | 6,386 ± 3,193 | 3,317 ± 1,436 | 16,198 ± 8,439 |
CFU-E | 1,707 ± 910 | 4,562 ± 1,645 | 3,731 ± 1,759 | 25,575 ± 9,645* |
BM and spleen cells from wild-type and KitV558∆;T669I/+ mice were cultured in the presence of KitL (100 ng/mL), EPO (6 U/mL), and IL-3 (100 ng/mL). CFU-E and BFU-E colonies were scored at the end of 2 and 10 d, respectively. n = 3 mice per group.
*P < 0.05 in comparison with wild type.