Table 1.
Injected clone | Live-born | T cell chimerism | T + B cell chimerism |
B-iPSC1 | 6 | 0 | 0 |
B-iPSC2 | 6 | 0 | 0 |
B-iPSC3 | 6 | 0 | 0 |
5aza-B-iPSC1 | 15 | 0 | 7 |
5aza-B-iPSC2 | 12 | 0 | 6 |
5aza-B-iPSC3 | 5 | 3 | 0 |
5aza-B-iPSC4 | 12 | 6 | 0 |
5aza-B-iPSC5 | 9 | 1 | 3 |
The total number of live-born mice and number of T and T + B-cell chimerism resulting from Rag2−/− blastocyst complementation with the indicated iPSC clones are shown. The presence of T and T + B-cell chimerism was determined by FACS analysis of Thy1.2 (T-cell)- and B220 (B-cell)-expressing cells in peripheral blood as shown in Fig. S3.