Table 5.
Completeness of transcripts and sequence conservation for some proteins involved in cellular spatial organization
| Query protein (GenBank Accession Number) | Amino acids in query sequence | Amino acids of query covered by best BLAST hit(s) | % amino-acid sequence identity (number of gaps) | Length ofAiptasiacontig (bp) | Positions in contig covered by best BLAST hit |
|---|---|---|---|---|---|
| Mouse Cdc42 (P60766) |
191 |
1-191 |
92 (0) |
1,399 |
275-847 |
| Mouse cyto-plasmic actin 1 (P60710) |
375 |
2-375 |
97 (0) |
1,455 |
117-1,247 |
| Mouse tubulin α1B (P05213) |
451 |
1-432 |
97 (0) |
1,971 |
1,879-584 |
| Mouse tubulin β5 (P99024) |
444 |
1-427 |
98 (0) |
2,592 |
1,529-249 |
| Mouse septin-2 (P42208) a |
361 |
139-329 |
65 (14b) |
1,979 |
3-617 |
| Mouse kinesin 1 heavy chain (Q61768) |
963 |
3-199 |
72 (0) |
906 |
592-2 |
| |
|
453-947 |
53 (14) |
2,138 |
6–1,514 |
| Mouse myosin 8 (P13542) |
1,937 |
23-1,903 |
50 (14) |
7,798 |
7,650-2,032 |
| Mouse dynein heavy chain 1 (Q9JHU4) |
4,644 |
21-216 |
53 (4) |
790 |
191-790 |
| 836-4,643 | 73 (30) | 11,600 | 3–11,414 |
a Our unpublished studies have shown that as expected, the A. pallida genome encodes multiple septins and that, like other septins [42], these contain the three conserved motifs of a GTP-binding site in their N-terminal regions. We do not yet know why none of these transcripts appears in full-length form in the current transcriptome.
b The predicted A. pallida protein has a single insertion of 14 amino acids near the C-terminus of the “septin-unique element” [42].