Table 2. Terminal restriction fragments (TRFs) predicted and produced from five target genes following individual species amplification and HhaI digestion.

	fusA	hly	recA		rpoB		prs		TRF Profile	Unique (total)
Species tested	DTRF	Uncut	UTRF	DTRF	UTRF	DTRF	UTRF	DTRF	(unique TRFs in bold)	TRFs
PREDICTED:
S. enterica	hex458*	–	–	–	rox226*	rox121*	–	–	hex456/rox121,226	3 (3)
L. monocytogenes	–	fam140*	fam288*	fam78	–	–	tet292	tet132	fam78,140,288/tet132/292	2 (5)
L. innocua	–	–	fam55	fam78	–	–	tet292	tet132	fam55,78/tet132/292	0 (4)
L. grayi	–	–	–	–	–	–	tet134*	tet132	tet132/134	1 (2)
L. ivanovii	–	–	fam55	fam78	–	–	tet127*	tet132	fam55,78/tet127/132	1 (4)
L. seeligeri	–	–	fam55	fam263*	–	–	tet292	tet292	fam55,263/tet292	1 (4)
L. welshimeri	–	–	fam103*	fam82*	–	–	tet265*	tet292	fam82,103/tet265/292	3 (4)
MEASURED:
S. enterica	hex453*	–	–	–	rox225*	rox117*	–	–	hex453/rox117,225	3 (3)
L. monocytogenes	–	fam138*	fam286*	fam73a	–	–	tet291	tet129b	fam73,138,286/tet129/291	2 (5)
L. innocua	–	–	fam51	fam73a	–	–	tet291	tet129b	fam51,73/tet129/291	0 (4)
L. grayi	–	–	–	–	–	–	tet133b	tet129b	tet129/133	1 (2)
L. ivanovii	–	–	fam51	fam73a	–	–	tet125b	tet129b	fam51,73/tet125/129	1 (4)
L. seeligeri	–	–	fam51	fam262*	–	–	tet291	tet129b	fam51,262/tet129/291	1 (4)
L. welshimeri	–	–	fam99*	fam75a	–	–	tet262*	tet288*	fam75,99/tet262/288	3 (4)

All TRFs are listed using the code: (dye)(length in bp).

^*TRFs unique to that species and definable on analysis.

^aTRFs combined into a single bin due to lack of distinction on analysis.

^bTRFs combined into a single bin due to lack of distinction on analysis.

UTRF: TRF derived from the upstream i.e. forward primer end of the amplicon.

DTRF: TRF derived from the downstream i.e. reverse primer end of the amplicon.