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. 2012 Aug 24;7(8):e43800. doi: 10.1371/journal.pone.0043800

Figure 1. Osteoblast differentiation induced by BMP-4 in iPS cells.

Figure 1

(A) Schematic representation of the osteoblast differentiation protocol for iPS cells by osteogenic cocktail. (B) Histochemical staining for ALP activity and with alizarin red in differentiated iPS cells on day 3 and day 15. EBs were grown for 15 days in the presence of Dex/h-GP/AA with BMP-4 and then stained (ALP and alizarin red). (C) Time course of osteoblast marker expression in osteoblastic differentiated iPS cells. Quantitative RT-PCR for osteoblast markers was carried out. Bars represent means ± SD of 3 separate wells.