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. 2012 Aug 24;7(8):e43711. doi: 10.1371/journal.pone.0043711

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© 2012 Liu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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AGS (A) and MKN45 (B) quiescent human gastric cancer cells and HUVECs (C) were treated with and without honokiol (HK, 5–40 µM) for the indicated times. Whole cell lysates were prepared and analyzed by Western blotting for detection of phosphorylated STAT-3 (pSTAT-3, Tyr705). The blottings were reprobed with anti-STAT-3 antibodies for normalization. The results are representative of at least five independent experiments.