Figure 4. Ecdysone receptors (EcR) and ultraspiracle (USP) proteins are under the control of 20-E.

(A) EcR and USP genes were quickly up-regulated when entering the wandering stage. The transcription levels of these genes were low during the feeding stage and the end of the wandering stage. (B–E) 20-E injection induced the transcription of EcR1, EcR3, and USP during the first 12 h compared with naive or buffer injection. EcR2 was up-regulated at 24 h after 20-E injection. *p<0.05; **p<0.001.