Skip to main content
. 2012 Aug 24;7(8):e43737. doi: 10.1371/journal.pone.0043737

Figure 2. Construction of the URA3 RNAi expression cassette plasmids used to silence URA3.

Figure 2

In all plasmids except for the dual promoter plasmid, URA3 transcription is driven by the glyceraldehyde-3-phosphate dehydrogenase promoter. pAN7-ura3-hp contains the antisense fragment of URA3 from 790-74 bp and the sense fragment from 306-790 bp (A). pAN7-ura3-s contains 484 bp of URA3 in the sense orientation (B). Complementary portions of the antisense and sense regions are paired in the 306-790 nucleotide region of the URA3 gene, forming a hairpin loop with a 484 bp complementary region (C). pAN7-ura3-as contains URA3 in the antisense orientation (D). Maps of the pAN7-1 vector and the inserted RNAi cassettes (E). The dual promoter plasmid contains 484 bp of URA3 between the gpd promoter and 35S promoter (F). The ura3-lcc co-silencing vector used the dual promoter method (G). Bar = 1000 bp