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URA3-silenced transformants (dual-2), wild-type (wt) and control transformants (CK1, transformed with the pAN7-1 plasmid) were cultured on MMM medium with or without uracil (100 mg/L) for 7 days at 28°C. The upper row (M+uracil) is MMM medium with uracil, and the lower row (MMM) is MMM medium.
