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. 2012 Aug 24;7(8):e43737. doi: 10.1371/journal.pone.0043737

Figure 7. Phenotypic assays of co-silenced transformants.

Figure 7

(A) URA3-lcc1 co-silenced transformants, wild-type and ck2 transformants were cultured on CYM medium containing 600 µg/mL 5-FOA for ten days. Wt represents the wild-type strain, ck2 strains contain the pAN7-ura3-dual vector, and lcc-1, 10, 12, 15, 16, and 17 represent ura3-lcc co-silenced transformants. (B) An ABTS-Petri dish was used to determine whether laccase was silenced in the co-silencing transformants. Lcc-1, lcc-10 lcc-12, lcc-15, lcc-16, and lcc-17 had significantly smaller colored circles compared with the wt and control strains.