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| Plasmid Configuration | Construction steps for further study | Replicate | silenced | non-silenced |
| Hairpin (pAN7-ura3-hp) | 2–3 | I | 7 | 4 |
| II | 8 | 7 | ||
| III | 9 | 5 | ||
| Mean(%) | 60.4 | 39.6 | ||
| Sense (pAN7-ura3-s) | 1 | I | 8 | 5 |
| II | 6 | 11 | ||
| III | 8 | 3 | ||
| Mean(%) | 56.5 | 43.5 | ||
| Antisense (pAN7-ura3-as) | 1 | I | 11 | 7 |
| II | 8 | 6 | ||
| III | 4 | 7 | ||
| Mean(%) | 51.5 | 48.5 | ||
| Dual-promoter (pAN7-ura3-dual) | 1 | I | 10 | 0 |
| II | 19 | 5 | ||
| III | 16 | 8 | ||
| Mean(%) | 81.9 | 18.1 | ||
| CK1 (pAN7-1) | 0 | I | 0 | 11 |
| II | 0 | 15 | ||
| III | 0 | 14 | ||
| Mean(%) | 0 | 100 |
