Table 1. Effects of G-CSF on mobilized bone marrow cells in the peripheral blood.
Hours afterCCI surgery | 0 | 3 | 6 | 12 | 24 | 48 | 72 | 144 | |||||||
Groups | Naïve | CCI+vehicle | CCI+G-CSF | CCI+vehicle | CCI+G-CSF | CCI+vehicle | CCI+G-CSF | CCI+vehicle | CCI+G-CSF | CCI+vehicle | CCI+G-CSF | CCI+vehicle | CCI+G-CSF | CCI+vehicle | CCI+G-CSF |
Total WBCs | 4.72 (0.23) | 6.04 (0.73) | 10.73 (1.06)* | 6.45 (0.81) | 11.11 (1.02)* | 8.72 (2.03) | 15.76 (2.22)* | 6.84 (0.82) | 13.95 (1.19)* | 6.31 (1.53) | 11.29 (0.67)* | 5.76 (0.98) | 10.88 (3.31) | 4.88 (0.75) | 5.80 (1.07) |
PMNs | 4.16 (0.18) | 5.18 (0.61) | 9.88 (1.10)* | 5.18 (0.70) | 10.43 (0.99)* | 7.81 (2.12) | 14.53 (2.36)* | 5.85 (0.87) | 12.51 (1.21)* | 4.71 (1.28) | 9.40 (0.64)* | 4.41 (1.11) | 9.55 (3.24) | 3.82 (0.60) | 4.48 (0.61) |
Lymphocytes | 0.41 (0.10) | 0.66 (0.13) | 0.69 (0.12) | 1.04 (0.28)* | 0.38 (0.08) | 0.58 (0.24) | 0.62 (0.35) | 0.58 (0.33) | 0.80 (0.26) | 1.38 (0.53) | 1.40 (0.33) | 1.09 (0.44) | 1.13 (0.88) | 1.15 (0.12) | 0.99 (0.35) |
Monocytes | 0.04 (0.02) | 0.09 (0.09) | 0.08 (0.07) | 0.10 (0.06) | 0.06 (0.04) | 0.08 (0.03) | 0.40 (0.28) | 0.30 (0.12) | 0.41 (0.09) | 0.16 (0.21) | 0.34 (0.14) | 0.15 (0.17) | 0.11 (0.22) | 0.39 (0.09) | 0.21 (0.14) |
Compared to vehicle treatment in rats with CCI, G-CSF treatment in rats with CCI significant increased the number of circulating WBCs and PMN cells at 3, 6, 12, 24, and 48 h after CCI. In contrast, the levels of circulating lymphocytes and monocytes at the above-mentioned time points were not significantly different between the G-CSF- and vehicle-treated rats with CCI, except at 6 h when there was a transient increase in the level of lymphocytes in vehicle group. Data are shown as the mean±SEM; n = 6 per group; Mann–Whitney U test, *p<0.05.