Figure 3. Triadin peptide modulates purified RyR1 single channel open time, closed time and closed frequency.

(A) Average data for open probability (P_o) in presence of triadin^200–232 and triadin^200–231 (n = 10), data from both peptides combined (see results text, collectively termed triadin peptide, n = 11–20) for each of the following parameters; open time (T_o), close time (T_c) and open frequency (F_o), collected at −40 mV and +40 mV. All data is expressed as relative mean data (Log rel (parameter)). Relative mean P_o (log rel P_o) is the average of differences between the log₁₀ of the P_o in the presence of either 63 nM or 252 nM triadin peptide (log₁₀ P_{o Pep}) and log₁₀ of the control P_o (log₁₀ P_{o Con}) for each channel. Log rel T_o is log₁₀ T_{o Pep}-log₁₀ T_{o Con}, log rel T_c is log₁₀ T_{c Pep}-log₁₀ T_{c Con} and log rel F_o is log₁₀ F_{o Pep}-log₁₀ F_{o Con}. (B) [³H]ryanodine binding to purified RyR1 in the absence and presence of 63 nM of triadin peptide. [³H]ryanodine binding is measured as pmol ryanodine/mg RyR1. Data is expressed relative to binding recorded in the absence of peptides (rel ryanodine binding). Significance (p≤0.05) is indicated for each concentration compared to activity recorded prior to addition of peptide (*).