Table 1. Quantitative real-time PCR primers.
Target group | Primer name | Primer sequence (5'-3') | Annealing (°C) | Primer conc. (nM) | Reference |
---|---|---|---|---|---|
Total bacteria |
1114f 1275r |
CGG CAA CGA GCG CAA CCC CCA TTG TAG CAC GTG TGT AGC C |
60 |
150 |
45 |
Lactobacillus group |
Lacto-F Lacto-R |
AGC AGT AGG GAA TCT TCC ACA CCG CTA CAC ATG GAG |
58 |
500 |
46 |
47 | |||||
Lactobacillus paracasei |
LcaseF LcaseR |
GCA CCG AGA TTC AAC ATG GGG TTC TTG GAT YTA TGC GGT ATT AG |
60 |
500 |
48 |
Lactobacillus rhamnosus |
LrhamF LcaseR |
TGC TTG CAT CTT GAT TTA ATT TTG GGT TCT TGG ATY TAT GCG GTA TTA G |
62 |
500 |
48 |
Bifidobacterium lactis | Bflact2F Bflact5R |
GTG GAG ACA CGG TTT CCC CAC ACC ACA CAA TCC AAT AC |
60 | 600 | 49 |