Figure 3. Analyses of neuritic extension of embryonic cortical neurons, embryonic viability and newborn weight.

A. Neuritic extension of cortical neurons from E14 embryos was analyzed after four days in culture by immunolabelling with anti-actetylated tubulin antibodies (bar: 20 µm). For each culture a minimum of 150 neurons were analyzed. Mean values for the total length of the neurites, the length of the longest neurite, the number of processes and the number of branches are presented. Error bars are standard errors of the mean. B. The genotype of 146 newborns from heterozygous matings was determined and genotype frequencies determined. C. The weight of neonate and of their brain was determined for 82 females and 64 males. The mean values for each genotype are presented.