Figure 1. Rumen digestion of unbound and soil-bound HY TME PrP^Sc.

(A and D): Representative immunoblots (n = 3–6) of HY TME in vitro rumen digestions. ‘B’: samples in McDougall's buffer with carbohydrates. ‘I’ samples in inactivated rumen buffer. ‘A’: samples in active rumen buffer. Incubation length was 20 hr at 39°C. All samples treated with proteinase K. (B, C, and E): Quantification of immunoblots. (B): Unbound (brain homogenate, BH), silty clay loam (SCL) Soil, and bentonite clay. (C): SiO2 powder. (E): Sand and sandy loam (SL) soil. All samples were normalized to the buffer 0 hr samples, except sand and SL soil samples (E) were normalized to the buffer 20 hr samples. Error bars show ±1 standard error of the mean.