Figure 1. MTORC1 regulates the subcellular distribution of TFEB. (A) Immunofluorescence confocal microscopy showing nuclear localization of recombinant TFEB-Flag in ARPE-19 cells incubated with MTORC1 inhibitors. (B) Quantification of the nuclear localization of TFEB in ARPE-19 cells incubated with different kinase inhibitors. Values are means ± SD of three independent experiments. (C) Immunofluorescence confocal microscopy showing colocalization between TFEB-Flag and MTORC1 in ARPE-19 cells incubated with PP242 for 2 h. (D) Quantification of the lysosomal localization of TFEB in ARPE-19 cells incubated with PP242. (E) Still images of a time-lapse movie showing the sequential association of TFEB-GFP with lysosomes and subsequent accumulation in the nucleus. Scale bars: 10 μm. ***p < 0.001; n.s.: not significant.