Figure 4. Dissociation of the TFEB/YWHA complex results in transport of TFEB to the nucleus and increased expression of autophagic and lysosomal genes. (A) Immunofluorescence confocal microscopy showing nuclear and late endosomal/lysosomal localization of TFEB-S211A in ARPE-19 cells. (B) Quantification of the nuclear localization of TFEB as shown in (A). Values are means ± SD of five independent experiments. (C–F) Relative quantitative RT-PCR analysis of the mRNA expression of autophagy (ATG9B and UVRAG) and lysosomal (LAMP1 and MCOLN1) genes in ARPE-19 cells infected with control adenovirus (Null) or adenovirus expressing TFEB-wt or TFEB-S211A. Values are means ± SD of three independent experiments. Scale bar: 10 μm. ***p < 0.001; **p < 0.01; *p < 0.05; n.s., not significant.