Figure 6. TFEB interacts with the MTORC1 complex. (A) HeLa (CF7) cells treated with either DMSO or Torin-1 (250 nM) for 1 h at 37°C were lysed and TFEB-Flag was immunoprecipitated using anti-FLAG antibody. Immunoprecipitates were then subjected to immunoblotting analysis using the indicated antibodies against components of the MTORC1 complex and RAB7. Note that inputs represent 3% of the total lysates and immunoprecipitated TFEB represents 10% of the total immunoprecipitated material. (B) Model depicting the proposed mechanistic regulation of TFEB by MTORC1.