Figure 6. Analysis of in vivo leukocyte histone acetylation by flow cytometry. Mean and median fluorescence values for histone H3 and histone H4 acetylation in four sets of leukocytes over a treatment course, obtained by flow cytometry. The standard error of the statistical mean is indicated by the error bars. The y-axis shows the relative fluorescence intensity. Pre-treatment patient data were histogram subtracted from that of cells collected 6, 24 and 48 h post a single 30 min infusion of 15 mg/m2 LBH589. Cells were fixed post-separation from blood by red cell lysis. The fixed cells were stained for histone H3 and histone H4 acetylation and analyzed by flow cytometry