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Figure 3. Activation of GLCE expression in MCF7 breast carcinoma cells by 5-aza-deoxycytidine or Trichostatin A. The intensity of the amplified GLCE DNA fragments was normalized to that of GAPDH (multiplex RT-PCR) and β-actin (Taqman-based qReal-Time RT-PCR). Bars represent the mean ± SD from triplicate experiments, **p < 0.01 – p values between MCF7 - TSA400-treated MCF7 and MCF7 - aza-TSA400-treated MCF7 data points (OriginPro 8.1). 5-aza-dC, 5-aza-deoxycytidine; TSA, Trichostatin A.
