Figure 4. Involvement of chromatin structure in GLCE activation upon TSA treatment. (A) Changes in the expression of different histone modifications in MCF7 cells after 5-aza-dC and TSA treatment. Western blot (WB) analysis with specific antibodies. (B) Chromatin immunoprecipitation assay for the GLCE promoter region. Chromatin DNA was immunoprecipitated with ChIP-grade antibodies and DNA fragments corresponding to 183 bp in the GLCE promoter region were amplified by PCR. The amount of immunoprecipitated DNA was normalized to that of the input DNA. *p < 0.05 – p values between MCF7 - aza-TSA400-treated MCF7 data points.