Figure 1. The methylation of miR-29a and miR-1256 promoters leads to decreased expression of miR-29a and miR-1256. Computerized analysis showed that there are many CpG sites in the promoters of miR-29a (A) and miR-1256 (B). Real-time RT-PCR showed that the expressions of miR-29a and miR-1256 were significantly downregulated in PCa cells [(C) *p < 0.05; n = 3] and in human prostate tumor tissues [(D) n = 46] compared with normal prostate epithelial cells and normal human prostate tissues.