Figure 2. The effects of isoflavone on miR-29a and miR-1256 expression. Methylation specific PCR showed that isoflavone or Aza-dC treatments could partly demethylate the promoters of miR-29a and miR-1256 (A). Real-time RT-PCR showed increased expression of miR-29a (B) and miR-1256 (C) after isoflavone or Aza-dC treatment. However, isoflavone and Aza-dC had differential effects on the expression of miR-155 and miR-421 (D). (C, control; A, Aza-dC; Iso, isoflavone; M, methylation; U, unmethylation)