Figure 3. Targeting TRIM68 by miR-29a and miR-1256. Computerized analysis showed miR-29a and miR-1256 sequence alignment to the sequence of TRIM68 3′UTR (A). Co-transfection of TRIM68 3′UTR-Luc and miR-29a, miR-1256 mimic, or negative control miRNA showed that miR-29a and miR-1256 directly bond to the 3′UTR of TRIM68 (B). Transfection of miR-29a, miR-1256 mimic, or negative control miRNA into PCa cells caused downregulation of TRIM68 and PGK-1 at mRNA (C) and protein (D) levels. (Neg: negative control miRNA; 29a: miR-29a; 1256: miR-1256; *: p < 0.05, n = 3)