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MDCK-SIAT1 cells were inoculated at a multiplicity of infection (MOI) of 0.001 PFU with the engineered viruses. Supernatants were harvested after at 12, 24, 36, 48, 60 and 72 hours post infection and were titrated on MDCK-SIAT1 cells. The results shown are from two independent experiments. Mean titers and standard deviations are shown.
