Figure 7. B-MYB target genes and possible interactions with pluripotency factors and regulation by histone methylation.
A) Overlap of target genes between B-MYB and other selected TFs (see materials and Methods). Numbers in bold and italic refer to significant overlaps between two gene groups with p<10−10, in bold refers to p<10−3, respectively. B) Overlap of binding sites for Oct4, Sox2, Nanog (OSN) and B-MYB, and the corresponding biological process (GO terms) that were significantly elevated among the overlapping genes. C) Transcription factor binding interactome of selected genes associated with pluripotency, cell cycle and epigenetic regulation. Each gene is indicated by a circle, and lines connecting genes indicate TF binding. TF binding is directional, as the color of the line indicates which factor binds to the connected gene promoter. The data indicate a high level of crosstalk among pluripotency, cell cycle and epigenetic regulators with B-Myb. Data were taken from Table S1. D) Overlap among B-MYB target genes with OSN (common targets of Oct4, Sox2 and Nanog identified in at least two ES cells) and the state of histone methylation (H3K4me3, H3K27me3). All target genes used in this analysis were differentially repressed by the B-MYB knockdown based on the microarray experiment.