Table 2. Primers for NNAT and genes in pathway expression detection.

Primer names	Sequences (5′-3′)	Products size (bp)	Melting temperature
NNAT	GGACTAGAGGGCGAGGGTGA	214	62°C
	GGCTTGGAGAAGGTGTTTTGGT
CAT2-Pig	ACAACTGGCGAAGAAGTCCG	100	65°C
	CTGCCGAGACCCCAAAATAG
GLUT1-Pig	GATGAAGGAGGAGTGCCG	106	68°C
	CAGCACCACGGCGATGAGGAT
GLUT3-Pig	TCACGGTCGGTTGAAATG	352	65°C
	CCCACAATCGCTGGAGGA
AKT1-Pig	GAAGCTGCTGGGCAAAGG	207	63°C
	CGGTCGTGGGTCTGGAA
HIF1A-Pig	ACAGAATGGAACGGAGCAAA	119	61°C
	CTTCACAATCATAACTGGTCA
IRS1-Pig	CAACCAGAGTGCCAAAGTGAT	83	61°C
	TGGGTGTCGAGGAGAAGGT
MTOR-Pig	CTGCTCATCAAACAAGCCACATC	116	62°C
	CAAACGCCTCCCGAGAACG
PI3K-Pig	CCAAGAGTTCCGTAAACAGTGCTA	196	63°C
	CTGCATGTAATGGACTGCCTCT
GAPDH-Pig	AAAGGCCATCACCATCTTCC	135
	GCCCCACCCTTCAAGTGAGCC
NNATpig2	GGCGGACTCCGAGAGACCA	280/199	58°C
	CCGGTTCTCGACACGGTGTA
NNAT-imprint	CAGCCCCTCACTGATCTTGAAT	117	56°C
	AATCTAGCCGGGGAGACA