Figure 1. T4-Stimulated Rac Expression is Associated with Increased Myocardial Rac-GTP Activity and Superoxide (O2·⁻) Production.

Western blot analyses of myocardial Rac1 (A) Representative images of Rac-GTP immunostaining in FFPE mid-ventricle sections, with bar graph for the mean intensity of the brown colored active Rac-GTP in arbitrary units (B & C). Representative images of myocardial O₂ ^·− generation with DHE in frozen sections of mice hearts, with bar graphs for mean fluorescence intensity in arbitrary units (D & E). * is significant change compared to untreated WT mice, ** is significant change compared to T4-treated and untreated WT mice, # is significant change compared to untreated transgenic mice, + is significant change compared to T4-treated transgenic mice, and ∧ is significant change compared to T4-supplemented transgenic mice that pre-treated with carvedilol (n = 8/group).