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. 2012 Aug 24;7(8):e42628. doi: 10.1371/journal.pone.0042628

Figure 2. ZNF750 mRNA and protein expression in Ca2+ induction and in human skin.

Figure 2

(A) QRT-PCR of ZNF750 mRNA expression during Ca2+ induction in NHEK cells. Filaggrin mRNA levels serve as internal control. Error bars represent mean values±SD, N = 3. (B) QRT-PCR of ZNF750 mRNA expression during Ca2+ induction in HaCaT cells. Error bars represent mean values±SD, N = 3. (C) ZNF750 protein levels during Ca2+ induction in HaCaT cells, analyzed by anti-ZNF750 antibodies against both N-terminal and C-terminal regions. (D) QRT-PCR of ZNF750, KRT1 and FLG mRNA expression in NHEK cells that were treated with PMA. Error bars represent mean values±SD, N = 2.