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. 2012 Aug 24;7(8):e43567. doi: 10.1371/journal.pone.0043567

Figure 3. Localization of vitellin in different phases of ovarioles during vitellogenesis of MEAM1 whiteflies.

Figure 3

The nuclear and vitellin were seen by laser scanning confocal microscopy following exposure to DAPI (blue) and FITC labeled anti-Vt antibody (green). I: previtellogenesis-phase I oocytes. More than 20 follicular cells (white arrow) surround the oocyte was observed. No signal of vitellin was detected. II: early vitellogenesis-phase II oocytes. Vitellin (white arrow) was observed around the follicular cell. III: mid vitellogenesis-phase III oocytes. Vitellin (white arrow) was formed spheres and filled most space of the oocyte. At the top of oocytes, a previtellogenesis oocyte was formed. A bacteriocyte sphere (showed with ‘B’) was uptake by the developmental oocyte. IV: post vitellogenesis-phase IV oocytes. Vitellin has filled with oocytes and formed mature oocytes. Egg pedicel (white arrow) was observed. The details of different phase of oocytes were described in Guo et al. [9]. Bars = 50 µm.