Table 3. Comparative table for the existing bacterial biosensors for phenol.
S.No. | Description | pRLuc42R Phenol Biosensor (This study) | Shingler et al.(1994) | Wise and kuske (2000) | Park et al. (2003) | Leedjarv et al. (2006) |
1. | Regulatory gene for Phenol+reporter gene | Mutated DmpR+luc | DmpR wt & DmpR-XylR hybrid +lux | DmpR+LacZ | CapR+Luc | DmpR+lux |
2. | Wild Type/Mutant | Both | Both | Both | Wild type | Wild type |
3. | Mutants (Positions) | Docking based at 42nd position of A domain | Random | Random | NA | NA |
Pollutants | ||||||
Priority pollutant Phenols(USEPA) | Detection Limit | Detection Limit | Detection Limit | Detection Limit | Detection Limit | |
I | Phenol | 500 nM | 3.2 µM | 2.5 µM | 10 µM | 0.082 mg/l (871 nM) |
II | 2-chlorophenol | 1 µM | 2.5 µM by Mutant | 100 µM | ||
III | 2, 4-dichlorophenol | 10 µM | 25 µM by Mutant | |||
IV | 2, 4, 6-trichlorophenol | 0.1 µM | Not Detected | |||
V | Pentachlorophenol | Not detected | Not Detected | |||
VI | 4-chloro-3-ethylphenol | 100 µM | 75 µM by Mutant | |||
VII | 2, 4-dimethylphenol | 0.1 µM | 75 µM by Mutant | 0.454 mg/l (3.71 µM) | ||
VIII | 2-nitrophenol | 100 µM | 75 µM by Mutant | 100 µM | ||
IX | 4-nitrophenol | 10 µM | 75 µM by Mutant | |||
X | 2, 4-dinitrophenol | Not detected | ||||
XI | 2-methyl-4,6-dinitrophenol | Not detected | Not Detected | |||
Other Phenolic derivatives | ||||||
A | Cresol & derivatives | 10 µM | 3.2 µM-2 mM | 100 µM | ||
Non Phenolic derivatives | ||||||
Xylene | Not detected |