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. 2012 Aug 24;7(8):e43527. doi: 10.1371/journal.pone.0043527

Table 3. Comparative table for the existing bacterial biosensors for phenol.

S.No. Description pRLuc42R Phenol Biosensor (This study) Shingler et al.(1994) Wise and kuske (2000) Park et al. (2003) Leedjarv et al. (2006)
1. Regulatory gene for Phenol+reporter gene Mutated DmpR+luc DmpR wt & DmpR-XylR hybrid +lux DmpR+LacZ CapR+Luc DmpR+lux
2. Wild Type/Mutant Both Both Both Wild type Wild type
3. Mutants (Positions) Docking based at 42nd position of A domain Random Random NA NA
Pollutants
Priority pollutant Phenols(USEPA) Detection Limit Detection Limit Detection Limit Detection Limit Detection Limit
I Phenol 500 nM 3.2 µM 2.5 µM 10 µM 0.082 mg/l (871 nM)
II 2-chlorophenol 1 µM 2.5 µM by Mutant 100 µM
III 2, 4-dichlorophenol 10 µM 25 µM by Mutant
IV 2, 4, 6-trichlorophenol 0.1 µM Not Detected
V Pentachlorophenol Not detected Not Detected
VI 4-chloro-3-ethylphenol 100 µM 75 µM by Mutant
VII 2, 4-dimethylphenol 0.1 µM 75 µM by Mutant 0.454 mg/l (3.71 µM)
VIII 2-nitrophenol 100 µM 75 µM by Mutant 100 µM
IX 4-nitrophenol 10 µM 75 µM by Mutant
X 2, 4-dinitrophenol Not detected
XI 2-methyl-4,6-dinitrophenol Not detected Not Detected
Other Phenolic derivatives
A Cresol & derivatives 10 µM 3.2 µM-2 mM 100 µM
Non Phenolic derivatives
Xylene Not detected