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Stoichiometry and Kinetics of Cyclohydrolase Reaction with DTNB. A.) PR-AMP cyclohydrolase complete reaction with DTNB. Conditions 50 mM Tris-HCl pH 8.5, 1mM EDTA, 5 mM MgCl2 30°C 625 μM DTNB, and 12.5 μM PR-AMP cyclohydrolase. B.) Stopped-flow results for the reaction of DTNB with native PR-AMP cyclohydrolase (solid line), cysteine (dots), and C109/116A (dashes). Conditions 50 mM KH2PO4 pH 7.3, 0.5 mM EDTA 1mM MgCl2 20ºC 440 μM DTNB 8.7 μM PR-AMP cyclohydrolase.
