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. Author manuscript; available in PMC: 2013 Jul 24.
Published in final edited form as: Biochemistry. 2012 Jul 9;51(29):5791–5803. doi: 10.1021/bi300391m

Table 2.

Zinc Content and Kinetics of PR-AMP Cyclohydrolase Preparations

Enzyme Species Zn2+ (moles/subunit) kcat/Km (M−1 s−1)
PR-AMP Cyclohydrolase 1.1, 0.88, 1.07 4.1 ± 0.8 × 105
D92E 0.72, 0.51 2.3 ± 0.2 × 103
C93A 0.003, 0.020 Inactive
D94A 0.60, 0.43 Inactive
D94E 0.65, 0.63 1.8 ± 0.2 × 102
C109A 0.37, 0.22 Inactive
H110A 1.16, 1.09 8.3 ± 2 × 102
C116A 0.99, 0.90 Inactive
C109/116A 0.027, 0.007 Inactive
DTNB or MMTS* 0.27, or 0.19*, 0.52*

The average percent deviation for the independent Zn2+ measurements is 10%.