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. Author manuscript; available in PMC: 2013 Jul 24.
Published in final edited form as: Biochemistry. 2012 Jul 9;51(29):5791–5803. doi: 10.1021/bi300391m

Table 4.

Stopped-Flow Comparison of DTNB reactions

Source Rate constanta (min−1) pH 7.3 Rate constanta (min−1) pH 8.5
Wild type 246 930
Cysteine 120 324
C109/116A 360 3384
a

Apparent first order rate constants. Conditions as described in experimental except as noted.