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. 1978 Nov;5(11):4155–4163. doi: 10.1093/nar/5.11.4155

Partial purification of transcriptionally active nucleosomes from trout testis cells.

B Levy, G H Dixon
PMCID: PMC342740  PMID: 569293

Abstract

Mononucleosomes (MN1) enriched in structural non-histone proteins and transcribed DNA sequences were obtained by limited digestion of trout testis nuclei with micrococcal nuclease followed by selective solubilization in 0.1 M NaCl. These monosomes consist of the four inner histones plus stoichiometric amounts of the non-histone protein H6, of the HMG group, complexed with 140 base pairs of DNA. Hybridization experiments indicate that MN1 DNA is enriched in sequences complementary to cytoplasmic polyadenylated RNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Elgin S. C., Weintraub H. Chromosomal proteins and chromatin structure. Annu Rev Biochem. 1975;44:725–774. doi: 10.1146/annurev.bi.44.070175.003453. [DOI] [PubMed] [Google Scholar]
  2. Felsenfeld G. Chromatin. Nature. 1978 Jan 12;271(5641):115–122. doi: 10.1038/271115a0. [DOI] [PubMed] [Google Scholar]
  3. Gottesfeld J. M., Butler P. J. Structure of transcriptionally-active chromatin subunits. Nucleic Acids Res. 1977 Sep;4(9):3155–3173. doi: 10.1093/nar/4.9.3155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gottesfeld J. M., Garrard W. T., Bagi G., Wilson R. F., Bonner J. Partial purification of the template-active fraction of chromatin: a preliminary report. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2193–2197. doi: 10.1073/pnas.71.6.2193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gottesfeld J. M. Methods for fractionation of chromatin into transcriptionally active and inactive segments. Methods Cell Biol. 1977;16:421–436. doi: 10.1016/s0091-679x(08)60117-x. [DOI] [PubMed] [Google Scholar]
  6. Gottesfeld J. M., Partington G. A. Distribution of messenger RNA-coding sequences in fractionated chromatin. Cell. 1977 Dec;12(4):953–962. doi: 10.1016/0092-8674(77)90160-x. [DOI] [PubMed] [Google Scholar]
  7. Hendrick D., Tolstoshev P., Randlett D. Enrichment for the globin coding region in a chromatin fraction from chick reticulocytes by endonuclease digestion. Gene. 1977;2(3-4):147–158. doi: 10.1016/0378-1119(77)90014-2. [DOI] [PubMed] [Google Scholar]
  8. Kornberg R. D. Structure of chromatin. Annu Rev Biochem. 1977;46:931–954. doi: 10.1146/annurev.bi.46.070177.004435. [DOI] [PubMed] [Google Scholar]
  9. Levy W B., Wong N. C., Dixon G. H. Selective association of the trout-specific H6 protein with chromatin regions susceptible to DNase I and DNase II: possible location of HMG-T in the spacer region between core nucleosomes. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2810–2814. doi: 10.1073/pnas.74.7.2810. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Levy B. W., Dixon G. H. Changes in the sequence diversity of polyadenylated cytoplasmic RNA during testis differentiation in rainbow trout (Salmo gairdnerii). Eur J Biochem. 1977 Mar 15;74(1):61–67. doi: 10.1111/j.1432-1033.1977.tb11366.x. [DOI] [PubMed] [Google Scholar]
  11. Levy B., Dixon G. H. Renaturation kinetics of cDNA complementary to cytoplamic polyadenylated RNA from rainbow trout testis. Accessibility of transcribed genes to pancreatic DNase. Nucleic Acids Res. 1977 Apr;4(4):883–898. doi: 10.1093/nar/4.4.883. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Maniatis T., Jeffrey A., van deSande H. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry. 1975 Aug 26;14(17):3787–3794. doi: 10.1021/bi00688a010. [DOI] [PubMed] [Google Scholar]

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