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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Nat Chem Biol. 2012 Jun 10;8(7):661–668. doi: 10.1038/nchembio.999

Figure 4. Mass spectrometry Analysis of N-glycans from Inhibitor treated cells.

Figure 4

HL-60 cells were treated for 7 days with DMSO only, 200 μM (2), 200 μM (8), or 200 μM each of (2) and (8). N-linked glycans from each sample were then isolated, permethylated and analyzed by MALDI-TOF MS. The four spectra represent treatment of the cells with (a) DMSO only, (b) the fucosyltransferase inhibitor (2), (c) the sialyltransferase inhibitor (8), or (d) both inhibitors in concert. All molecular ions are [M+Na]+. Putative structures are based on composition, tandem MS, and biosynthetic knowledge. Structures that show sugars outside a bracket have not been unequivocally defined.